Analysis Study of Stevioside and Rebaudioside A from Stevia rebaudiana Bertoni by Normal Phase SPE and RP-HPLC
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چکیده
منابع مشابه
Determination of Rebaudioside A and Stevioside in Leaves of S. rebaudiana Bertoni Grown in México by a Validated HPLC Method
Stevia rebaudiana is a plant with high sweetening capacity due to its content of glycosides, mainly stevioside and rebaudioside A. Several techniques have been used to determine the concentrations of glycosides in Stevia, although an HPLC method is recommended by the FAO/WHO-JECFA. Varieties of Stevia have been recently grown in Mexico, with no previous report of glycosides by a validated metho...
متن کاملMutagenicity and human chromosomal effect of stevioside, a sweetener from Stevia rebaudiana Bertoni.
Leaves of Stevia rebaudiana Bertoni have been popularly used as a sweetener in foods and beverages for diabetics and obese people due to their potent sweetener stevioside. In this report, stevioside and steviol were tested for mutagenicity in Salmonella typhimurium strains TA98 and TA100 and for chromosomal effects on cultured human lymphocytes. Stevioside was not mutagenic at concentrations up...
متن کاملHPTLC method for simultaneous analysis of stevioside and rebaudioside-A in Stevia rebaudiana.
An HPTLC method was developed and validated for simultaneous determination of stevioside (STE) and rebaudioside-A (REB-A) in Stevia rebaudiana leaves. The HPTLC separation was performed on precoated silica gel 60F254 HPTLC plates with the mobile phase ethyl acetate-ethanol-acetone-water (15 + 3 + 6 + 6, v/v/v/v). The densitometric quantification of steviol glycosides was carried out at Amax 580...
متن کاملAssessment of genetic and biochemical diversity of Stevia rebaudiana Bertoni by DNA fingerprinting and HPLC analysis
The genomic DNA polymorphism and phytochemical variation of Stevia rebaudiana Bertoni was investigated by RAPD-PCR and HPLC analysis. For initial PCR screening, about hundred oligo-nucleotide primers were used to amplify the genomic DNA from three accessions of S. rebaudiana (L1 to L3). The concentration of stevioside content was quantified by using HPLC analysis. A total of 9 primers were fina...
متن کاملPurple Phototrophic Bacterium Enhances Stevioside Yield by Stevia rebaudiana Bertoni via Foliar Spray and Rhizosphere Irrigation
This study was conducted to compare the effects of foliar spray and rhizosphere irrigation with purple phototrophic bacteria (PPB) on growth and stevioside (ST) yield of Stevia. rebaudiana. The S. rebaudiana plants were treated by foliar spray, rhizosphere irrigation, and spray plus irrigation with PPB for 10 days, respectively. All treatments enhanced growth of S. rebaudiana, and the foliar me...
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ژورنال
عنوان ژورنال: IOP Conference Series: Materials Science and Engineering
سال: 2018
ISSN: 1757-8981,1757-899X
DOI: 10.1088/1757-899x/349/1/012071